离子色谱-柱后衍生可见光检测法测定海水中痕量六价铬
赵云芝,庞妍娇,张璐涛,任朝辉,黄玲
四川省生态环境监测总站,成都 610091
Determination of trace hexavalent chromium in sea water by ion chromatography-post column derivatization with UV detection
ZHAO Yunzhi, PANG Yanjiao, ZHANG Lutao, REN Zhaohui, HUANG Ling
Sichuan Ecological and Environmental Monitoring Center, Chengdu 610091, China
摘要:建立了银柱和钠柱串联净化-离子色谱-柱后衍生可见光检测法测定海水中痕量六价铬的分析方法。海水样品经稀释后通过银柱和钠柱串联净化,以 Ion PacTM AS77 柱(250 mm× 2 mm)为分离柱,硫酸铵-氨水溶液为淋洗液,二苯羰酰二肼溶液为显色液,海水中六价铬经分离后与显色液在混合反应圈中进行衍生反应,衍生物经可见光检测器检测。六价铬的质量浓度在 0.1~10.0 μg/L 范围内与色谱峰面积呈良好的线性关系,线性相关系数为 0.999 9。当进样体积为 200 μL 时,方法检出限为 0.03 μg/L。 海水中六价铬的平均加标回收率为 78.0%,测定结果的相对标准偏差为2.4%n=6)。该方法能够满足海水中痕量六价铬的测定要求。
Abstract:An analytical method was developed for the determination of trace hexavalent chromium in sea water by purification with silver column and sodium column-ion chromatography-post column derivatization with UV detection. The sea water samples were diluted and purified with silver column and sodium column. The separation of hexavalent chromium was performed on a Ion PacTM AS77 column (250 mm× 2 mm) using ammonium sulphate-ammonia solution. Hexavalent chromium in sea water was derived with diphenylcarbonyl dihydrazine solution in mixed reaction ring. The detection was achieved with UV detector. The mass concentration of hexavalent chromium had a good linear relationship with the chromatographic peak area in the range of 0.1-10.0 μg/L, the correlation coefficient was 0.999 9. When the injection volume was 200 μL, the detection limits was 0.03 μg/L. The average recovery of hexavalent chromium in seawater was 78.0%, and the relative standard deviations of the determination results were 2.4% (n=6). The method can meet the requirement of determination of trace hexavalent chromium in sea water.
关键词:痕量六价铬;海水;银柱和钠柱串联净化;离子色谱-柱后衍生可见光检测法
Keywords:trace hexavalent chromium; sea water; purification with silver column and sodium column; ionchromatography-post column derivatization with UV detection
本文引用格式:
赵云芝,庞妍娇,张璐涛,等.离子色谱-柱后衍生可见光检测法测定海水中痕量六价铬[J].化学分析计量,2023,32(5):73.
ZHAO Yunzhi,PANG Yanjiao,ZHANG Lutao,et al. Determination of trace hexavalent chromium in sea water by ion chromatography-post column derivatization with UV detection[J]. Chemical Analysis and Meterage,2023,32(5):73.
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