收藏我们   ENGLISH  
论文查询
范围:  
提 交
《化学分析计量》 2023年第05期
DOI:10.3969/j.issn.1008-6145.2023.05.012
高效液相色谱串联质谱法测定动物源性食品中苯佐卡因、利多卡因
陆静,申甜甜,焦艳娜,梁香,朱绍华,刘紫涵,夏雨桑,付善良,成婧
长沙海关技术中心,食品安全科学技术湖南省重点实验室,长沙 410004 
Determination of benzocaine and lidocaine residues in animal derived foods by HPLC-MS/MS
LU Jing, SHEN Tiantian, JIAO Yanna, LIANG Xiang, ZHU Shaohua, LIU Zihan, Xia Yusang, FU Shanliang, CHENG Jing
Technology Center of Changsha Customs, Hunan Key Laboratory of Food Safety Science & Technology, Changsha 410004, China 
摘要:建立高效液相色谱串联质谱法检测动物源性食品中的苯佐卡因、利多卡因残留量的分析方法。 称取粉碎后的样品 5.0 g,置于 50 mL 离心管中,加入 1 g NaCl,10 mL 乙腈,振荡提取 20 min 后,以 4 500 r/min 转速离心 5 min,取上清液 6 mL,分别加入乙二胺 -N-丙基硅烷(pPSA) 50 mg 、C 50 mg,然后在涡旋仪上涡旋 1 min,以 4 500 r/min 转速 18 ∶ 离心 5 min,然后取 0.5 mL 上清液与 0.5 mL 复溶液[0.1% 甲酸-乙腈(体积比为 80 20)混合液]混合,过 0.22 μm 滤膜,供高效液相色谱串联质谱法测定。以 0.1% 乙酸-乙腈为流动相,C 柱为色谱柱,多反应监测模式分析,色谱峰面积外 18 标法定量。苯佐卡因和利多卡因在 0.1~50.0 μg/L 的质量浓度范围内线性关系良好,相关系数均大于 0.999,检出限分别为 0.2、0.1 μg/kg,定量限分别为 0.7、0.5 μg/kg,回收率分别为 85.0%~100.6%、87.7%~99.8%,5 次测定结果的相对标准偏差分别为 0.3%~3.5%、1.8%~3.6%。该方法可以满足动物源性食品中苯佐卡因、利多卡因同时检测的需要。 
Abstract:A method was established for the determination of benzocaine and lidocaine in animal derived foods by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). 5.00 g of sample was put into a 50 mL centrifuge tube with 1 g sodium chloride, then 10 mL acetonitrile was added as extraction solvent, and shaken for 20 min. The sample tubes centrifuged at 4 500 r/min for 5 min. Then 50 mg octadecyl trimethoxysilane powder (C ) and 50 18 mg n-propyl ethylenediamine powder (PSA) were added into 6 mL of the supernatant, the mixture was mixed well by vortex for 1 min. The sample tubes centrifuged at 5 500 r/min for 5 min, then mixed 0.5 mL of supernatant with 0.5 mL of ∶ complex solution [0.1% formic acid-acetonitrile (80 20, volume ratio)] and analyzed by HPLC-MS/MS after filtered through a 0.22 μm membrane. Gradient elution was performed by using acetonitrile and 0.1% acetic acid. The mass spectrometric acquisition was carried out by means of multiple reaction monitoring (MRM) after separated by C column. 18 Good linearity was obtained in the range of 0.1-50.0 μg/L for benzocaine and lidocaine, the correlation coefficient were more than 0.999. The limits of detection (LOD) for benzocaine and lidocaine were 0.2, 0.1 μg/kg, respectively. The limits of quantitation (LOQ) for benzocaine and lidocaine were 0.7, 0.5 μg/kg, respectively. The recoveries were 85.0%-100.6% and 87.7%-99.8%, respectively. The relative standard deviations of the five determinations were 0.3%-3.5% and 1.8%-3.6%, respectively. This method is suitable for the determination of benzocaine and lidocaine in animal derived foods. 
关键词:高效液相色谱串联质谱法;苯佐卡因;利多卡因;动物源性食品 
Keywords:HPLC-MS/MS; benzocaine; lidocaine; animal derived foods 
基金:海关总署科技计划项目(2021HK202)、长沙海关科技微创新项目(2021) 
本文引用格式:
陆静,申甜甜,焦艳娜,等.高效液相色谱串联质谱法测定动物源性食品中苯佐卡因、利多卡因[J].化学分析计量,2023,32(5):56.
LU Jing,SHEN Tiantian,JIAO Yanna,et al. Determination of benzocaine and lidocaine residues in animal derived foods by HPLC-MS/MS[J]. Chemical Analysis and Meterage,2023,32(5):56. 

全文:
相似文章:
  高效液相色谱串联质谱法测定水产品中氟喹诺酮类药物残留的不确定度评定
2023年第05期
  高效液相色谱串联质谱法同时测定化妆品中苔黑醛和氯化苔黑醛
2023年第01期
  高效液相色谱串联质谱法测定盐酸雷尼替丁胶囊中N-亚硝基二甲胺
2022年第02期
  固相萃取-液相色谱串联质谱法测定食品中的麻黄碱、芬氟拉明、西布曲明和酚酞
2022年第02期
  超高效液相色谱串联质谱法测定蜂蜜中氯霉素、氟甲砜霉素、甲砜霉素、甲硝唑
2021年第12期
  超高效液相色谱串联质谱法测定木贼中4个吡咯里西啶生物碱
2021年第12期
  高效液相色谱-串联质谱内标法测定动物源性食品中玉米赤霉烯酮及其代谢产物
2021年第01期
  超高效液相色谱-串联质谱法快速测定粮谷中的玉米赤酶烯酮
2018年第05期
  直接进样-超高效液相色谱串联质谱法同时测定水源水中9种农药
2016年第01期
  HPLC-MS/MS法测定南美白对虾苗中硝基呋喃类代谢物残留
2014年第01期
《化学分析计量》杂志社 版权所有,未经授权禁止复制或者建立镜像
地址:山东省济南市天桥区田庄东路3号 邮编:250031
电话:0531-85878132 85878224 85878278
鲁ICP备05018524号-6