收藏我们   ENGLISH  
论文查询
范围:  
提 交
《化学分析计量》 2023年第05期
DOI:10.3969/j.issn.1008-6145.2023.05.009
高效液相色谱法测定马来酸麦角新碱注射液中甲硫氨酸
盖成,苗会娟,徐艳梅,郝丽娟,高燕霞
河北省药品医疗器械检验研究院,石家庄 050200 
Determination of methionine in ergometrine maleate injection by high performance liquid chromatography
GE Cheng, MIAO Huijuan, XU Yanmei, HAO Lijuan, GAO Yanxia
Hebei Provincial Institute of Drug and Medical Device Inspection, Shijiazhuang 050200, China 
摘要:建立高效液相色谱法测定马来酸麦角新碱注射液中甲硫氨酸含量的方法。 采用 Venusil HILIC 色谱柱(250 mm×4.6 mm, 5 μm),以 5 mmol/L 磷酸氢二钾溶液(用磷酸调节 pH 值至 6.9)-乙腈(体积比为 20∶80)为流动相,流量为 1.0 mL/min,柱温为 30 ℃,检测波长为 215 nm。结果表明,甲硫氨酸质量浓度在 20~400 μg/mL 范围内与色谱峰面积的线性关系良好,相关系数为 0.999 9,检出限和定量限分别为 0.5、1.6 μg/mL,平均加标回收率为 99.7%,测定结果的相对标准偏差为 0.3%(n=6)。该方法适用于测定马来酸麦角新碱注射液中甲硫氨酸的含量。 
Abstract:A high performance liquid chromatography method was established to determine the methionine in ergometrine maleate injection. The method was carried out on Venusil HILIC column (250 mm×4.6 mm, 5 μm), and 5 mmol/L dipotassium hydrogen phosphate aqueous solution (pH was adjusted to 6.9 with phosphoric acid) - acetonitrile ∶ (volume ratio of 20 80) was used as mobile phase at a flow rate of 1.0 mL/min. The column temperature was 30 ℃, and the detection wavelength was 215 nm. There was a good linear relationship for methionine in the range of 20-400 μg/mL with the correlation coefficient of 0.999 9, the limit of detection and limit of quantification were 0.5 and 1.6 μg/mL, respectively. The average recovery rate of the test sample was 99.7%, the relative standard deviation of the measurement results was 0.3% (n=6). The established method is suitable for the determination of the content of methionine in ergometrine maleate 
关键词:甲硫氨酸;亲水作用色谱;高效液相色谱法 
Keywords:methionine; hydrophilicity chromatography; high performance liquid chromatography 
本文引用格式:
盖成,苗会娟,徐艳梅,等.高效液相色谱法测定马来酸麦角新碱注射液中甲硫氨酸[J].化学分析计量,2023,32(5):43.
GE Cheng,MIAO Huijuan,XU Yanmei,et al. Determination of methionine in ergometrine maleate injection by high performance liquidchromatography[J]. Chemical Analysis and Meterage,2023,32(5):43. 

全文:
相似文章:
  电喷雾检测器高效液相色谱法测定复方北豆根氨酚那敏片中灰毡毛忍冬皂苷乙和川续断皂苷乙
2024年第01期
  高效液相色谱法同时测定明胶空心胶囊中苯甲酸和山梨酸
2024年第01期
  高效液相色谱法测定化妆品原料中N-乙酰神经氨酸
2024年第01期
  高效液相色谱法测定诃子中诃黎勒酸和诃子酸
2024年第01期
  多功能针式过滤器净化超高效液相色谱法测定大米和花生中玉米赤霉烯酮
2024年第01期
  柱前衍生-高效液相色谱法测定脑蛋白水解物中多肽含量
2023年第11期
  高效液相色谱法测定化妆品中5种水杨酸酯紫外吸收剂
2023年第11期
  高效液相色谱法测定喘舒片中的硫及其结果的聚类分析
2023年第11期
  限进介质色谱柱高效液相色谱法测定动物源性食品中对乙酰氨基酚残留量
2023年第11期
  高效液相色谱法测定冷面、米线中乙二胺四乙酸二钠
2023年第10期
《化学分析计量》杂志社 版权所有,未经授权禁止复制或者建立镜像
地址:山东省济南市天桥区田庄东路3号 邮编:250031
电话:0531-85878132 85878224 85878278
鲁ICP备05018524号-6