超高效液相色谱指纹图谱结合化学计量学优选掌叶大黄饮片炮制工艺
张正,施文婷,莫秋怡,曹嵌,邓桂海,田清清,梁志毅
广东一方制药有限公司,广东省中药配方颗粒企业重点实验室,广东佛山 528244
Optimization of the processing technology of Rheum Palmatum Linn. pieces by ultra-high performance liquid chromatography fingerprint and chemometrics
ZHANG Zheng, SHI Wenting, MO Qiuyi, CAO Qian, DENG Guihai, TIAN Qingqing, LIANG Zhiyi
Guangdong Yi Fang Pharmaceutical Co., Ltd, Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Foshan 528244, China
摘要:优选掌叶大黄饮片的炮制工艺。建立基于UPLC(超高效液相色谱)的掌叶大黄饮片主要指标成分指纹图谱测定方法,结合相似度评价、聚类分析、主成分分析和方差分析等化学计量学方法,对不同润制时间、不同干燥方式、不同烘干温度及时间掌叶大黄饮片的指纹图谱信息进行分析。不同润制时间掌叶大黄饮片的指纹图谱之间存在明显差异;聚类分析可将不同润制时间的掌叶大黄饮片分为2类;主成分分析发现两个特征值均大于1的主成分包含了原始数据94.9%的信息;方差分析结果表明干燥方式、烘干温度和时间对掌叶大黄饮片的主要成分含量无明显影响。优选的掌叶大黄饮片炮制工艺:润制36 h,切厚片, 50~70℃下干燥8~12 h。本研究可为掌叶大黄饮片的规范化炮制生产提供依据。
Abstract:The processing technology of Rheum Palmatum Linn. pieces was optimized. A ultra-high performance liquid chromatography-based ?ngerprint analysis method for the main index components of Rheum Palmatum Linn. pieces was established, combined with chemometric methods such as similarity evaluation, cluster analysis, principal component analysis (PCA), and variance analysis, the fingerprint information of the Rheum Palmatum Linn. pieces of different moistening time, di?erent drying methods and di?erent drying temperature and time were analyzed. There were signi?cant di?erences in the ?ngerprints of Rheum Palmatum Linn. pieces at di?erent moistening times. Cluster analysis could classify Rheum Palmatum Linn. pieces with di ?erent moistening times into two categories. Principal component analysis found that the two principal components with eigenvalues greater than 1 contained 94.9% of the original data. The results of the analysis of variance showed that the drying methods, drying temperature and time had no signi?cant e?ect on the content of the main components of Rheum Palmatum Linn. pieces. The optimal processing technology of Rheum Palmatum Linn. pieces is as follows: moistening for 36 hours, cutting into thick slices, and drying at 50-70℃for 8-12 hours. This study can provide a basis for the standardized processing and production of Rheum Palmatum Linn. pieces.
关键词:掌叶大黄;超高效液相色谱指纹图谱;炮制工艺;相似度评价;聚类分析;主成分分析;综合评分;方差分析
Keywords:Rheum Palmatum Linn.; ultra-high performance liquid chromatography fingerprint; processingtechnology; similarity evaluation; cluster analysis; principal component analysis; comprehensive score; variance analysis
基金:广东省科技计划项目(2018B030323004, 2020A050515001);香港科技大学南海创新中心项目(FSUS719–SRI10)
本文引用格式:
张正,施文婷,莫秋怡,等.超高效液相色谱指纹图谱结合化学计量学优选掌叶大黄饮片炮制工艺[J].化学分析计量,2022,31(4):33.
ZHANG Zheng,SHI Wenting,MO Qiuyi,et al. Optimization of the processing technology of Rheum Palmatum Linn. pieces based on ultra-high performance liquid chromatography ?ngerprint and chemometrics[J]. Chemical analysis and meterage,2022,31(4):33.
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