柱前在线衍生-高效液相色谱法同时测定复方丹参胶囊中19 种氨基酸
许有诚,赵庄,李丹凤
(广西壮族自治区食品药品检验所,南宁 530021)
Simultaneous determination of 19 amino acids in Composite Salvia Miltiorrhiza Capsules by high performance liquid chromatography with online pre-column derivatization
许有诚,赵庄,李丹凤
(Guangxi Institute For Food and Drug Control, Nanning 530021, China)
摘要:建立柱前在线衍生-高效液相色谱法同时测定复方丹参胶囊中19 种氨基酸含量的方法。采用Agilent
HPH-C18(4.6 mm×150 mm,2.7 μm)色谱柱,以10 mmol/L 硼酸钠-磷酸氢二钠缓冲液(用6 mol/L 盐酸调节pH 值至8.2)为流动相A,乙腈-甲醇-水(体积比为41∶41∶18)溶液为流动相B,梯度洗脱,流量为1.0 mL/min,柱温为40 ℃,检测波长分别为338 nm 和262 nm,进样体积为1 μL。19 种氨基酸在各自的质量浓度范围内与色谱峰面积呈良好的线性关系,相关系数为0.999 4~0.999 9,方法检出限为0.06~0.68 μg/mL。样品加标回收率为91.4%~106.5%,测定结果的相对标准偏差为0.1%~1.8%(n =6)。该方法可用于复方丹参胶囊中多种氨基酸的同时测定。
Abstract:A method of the simultaneous determination of 19 amino acids contents in Composite Salvia Miltiorrhiza
Capsules by high performance liquid chromatography with online pre-column derivatization was established. The
chromatographic column was Agilent HPH-C18(4.6 mm×150 mm, 2.7 μm). The mobile phase A was 10 mmol/L
sodium borate-disodium hydrogen phosphate buffer(adjusted to pH 8.2 with 6 mol/L hydrochloric acid), and the mobile
phase B was acetonitrile-methanol-water(the volume ratio was 41∶41∶18) with gradient elution. The flow rate was 1.0 mL/min, the column temperature was 40 ℃, the detection wavelength was 338 nm and 262 nm, and the injection volume
was 1 μL. The mass concentration of 19 amino acids had a good linear relationship with the chromatographic peak area in
their respective range, the correlation coefficients were 0.999 4-0.999 9, and the method detection limits were 0.06-0.68
μg/mL. The average recoveries were 91.4%-106.5%, and the relative standard deviations of determination results were
0.1%-1.8%(n =6). The method can be used for the simultaneous determination of multiple amino acids in Composite Salvia
Miltiorrhiza Capsules.
关键词:复方丹参胶囊;氨基酸;柱前衍生;高效液相色谱法
Keywords:Composite Salvia Miltiorrhiza Capsules; amino acid; pre-column derivatization; high performance liquid chromatography
基金:广西科技计划(重点研发计划)项目(桂科AB18221125)
本文引用格式:
许有诚,赵庄,李丹凤. 柱前在线衍生- 高效液相色谱法同时测定复方丹参胶囊中19 种氨基酸[J]. 化学分析计量,2021,30(10):1.
Xu Youcheng,ZhaoZhuang,Li Danfeng. Simultaneous determination of 19 amino acids in Composite Salvia Miltiorrhiza Capsules by high performance liquid chromatography with online pre-column derivatization[J]. Chemical Analysis and Meterage,2021,30(10):1.
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