近红外光谱法快速分析降糖中成药中盐酸二甲双胍、格列齐特
郑凤玲,刘莉,黄双,白俊杰,李春美,陈娜,谭超
宜宾学院,过程分析与控制四川省高校重点实验室,四川宜宾 644000
Rapid determination of metformin hydrochloride and gliclazide in antidiabetic of traditional Chinese medicine by near infrared spectroscopy
郑凤玲,刘莉,黄双,白俊杰,李春美,陈娜,谭超
Process Analysis and Control of Sichuan University Key Laboratory, Yibin University, Yibin 644000, China
摘要:建立近红外光谱-偏最小二乘法分析降糖宁胶囊中盐酸二甲双胍、格列齐特的含量。以一定的浓度梯度配制掺杂样品,采用积分球漫反射方式,波数范围为4 000~10 000 cm-1,分辨率为8 cm-1,单个样品扫描32次,再运用原始数据形式处理、光谱平滑处理来优化模型。运用偏最小二乘法对掺杂样品进行线性拟合和偏差分析,结果表明:掺盐酸二甲双胍样品相关系数为0.996 2,偏差为-0.03~0.01;掺格列齐特样品相关系数为0.996 0,偏差为-0.001 1~0.001 5。掺杂样品浓度的预测值与实测值具有良好的线性关系且偏差小,准确度高,未知样量化分析的拟合值高。该法可用于快速分析降糖中成药中盐酸二甲双胍、格列齐特的掺杂含量。
Abstract:A near-infrared spectroscopy combined with partial least squares (PLS) method was established to analyze the adulterate concentration of metformin hydrochloride and gliclazide in Jiangtangning. Adulterate samples were prepared with a certain concentration gradient, and the detection wavelength was 4 000-10 000 cm-1 with a distinguishability of 8 cm-1. A single sample was scanned 32 times, and then the model was optimized by raw data format and spectral smoothing processing. The linear fitting and deviation analysis of the adulterate samples were carried out by PLS method. The results showed that the correlation coefficient of the samples adulterants with metformin hydrochloride was 0.996 2, and the deviation was -0.03-0.01; the correlation coefficient of gliclazide adulterants samples was 0.996 0, and the deviation was -0.001 1-0.001 5. There is a good linear relationship between predicted values and measured values of adulterate sample concentration, and the deviation range is small, the accuracy is high, and the fitting value of unknown sample quantitative analysis is high. The method can be used to quickly analyze the two adulterants in hypoglycemic Chinese patent medicines.
关键词:近红外光谱;快速分析;降糖中成药;偏最小二乘法;盐酸二甲双胍;格列齐特
Keywords:near-infrared spectroscopy; rapid analysis; antidiabetic traditional Chinese medicine; partial least squares; metformin hydrochloride; gliclazide
基金:四川省省级大学生创新创业训练计划项目(S201910641061)
本文引用格式:
郑凤玲,刘莉,黄双,等.近红外光谱法快速分析降糖中成药中盐酸二甲双胍、格列齐特[J].化学分析计量,2021,30(8):34.Zheng Fengling,Liu Li,Huang Shuang,et al. Rapid determination of metformin hydrochloride and gliclazide in antidiabetic of traditional chinese medicine by near infrared spectroscopy[J]. Chemical Analysis and Meterage,2021,30(8):34.
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