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《化学分析计量》 2021年第02期
DOI:10.3969/j.issn.1008-6145.2021.02.007
超高效液相色谱法测定环境空气中13 种醛酮类化合物
尹燕敏,刘淼,孙欣阳,邹强
苏州环境监测中心,江苏苏州 215004 
Determination of 13 aldehydes and ketones in ambient air by ultra performance liquid chromatography
Yin Yanmin, Liu Miao, Sun Xinyang, Zou Qiang
Suzhou Environmental Monitoring Central of Jiangsu Province, Suzhou 215004, China 
摘要:建立了一种超高效液相色谱法快速同时测定环境空气中13 种醛酮类化合物。采集环境空气中醛酮类化合物,与2,4-二硝基苯肼(DNPH)反应生成腙类衍生物,以乙腈-水为流动相,采用梯度洗脱,流量为0.4 mL/min,二极管阵列检测器检测。在优化的色谱条件下,13 种醛酮类衍生物的质量浓度在各自线性范围内与色谱峰面积线性关系良好,相关系数均大于0.995,检出限为0.019~0.030 mg/L。若采样体积为50 L,环境空气中13 种醛酮类化合物的最低检测浓度为0.15~0.28 nmol/mol。实际样品的加标回收率为79.4%~106%,测定结果的相对标准偏差为1.1%~6.9%(n=6)。该法简便、快速,可以满足环境空气中痕量醛酮类化合物的分析。 
Abstract:An ultra performance liquid chromatography (UPLC) method was developed for the simultaneous determination of 13 aldehydes and ketones in ambient air. Aldehydes and ketones in ambient air were collected and reacted with 2,4-dinitrophenylhydrazine (DNPH) to form hydrazone derivatives. Acetonitrile water was used as mobile phase, gradient elution was used, and the flow rate was 0.4 mL/min. The detection was performed by diode array detector. Under the optimized chromatographic conditions, the linear relationship between the mass concentration of 13 aldehydes and ketone derivatives and the peak area was good, the correlation coefficients were all greater than 0.995, and the detection limits were 0.019-0.030 mg/L. The minimum detection concentration of 13 aldehydes and ketones in the ambient air was 0.15-0.28 nmol/mol when the sample volume was 50 L. The recoveries were 79.4%-106% and the relative standard deviations were 1.1%-6.9% (n=6). The method is simple, rapid, it can be used for the analysis of trace aldehydes and ketones in ambient air. 
关键词:环境空气;醛酮类化合物;2,4-二硝基苯肼;超高效液相色谱 
Keywords:ambient air; aldehydes and ketones; 2,4-dinitrophenylhydrazine (DNPH); ultra performance liquid chromatography 
基金:苏州市环保科技项目(环境空气中有机物的监测技术研究(B201901) 
本文引用格式:
尹燕敏,刘淼,孙欣阳,等. 超高效液相色谱法测定环境空气中13 种醛酮类化合物[J]. 化学分析计量,2021,30(2):32.
Yin Yanmin,Liu Miao,Sun Xinyang,et al. Determination of 13 aldehydes and ketones in ambient air by ultra performance liquid chromatography[J]. Chemical Analysis and Meterage,2021,30(2):32. 

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