高效液相色谱法定量分析奥曲肽
向童欣,黄永鹏,唐慧,钟辉,陈博
军事科学院防化研究院,北京 102205
Quantitative analysis of octreotide by high performance liquid chromatography
向童欣,黄永鹏,唐慧,钟辉,陈博
Research Institute of Chemical Defense, Beijing 102205, China
摘要:建立奥曲肽的高效液相色谱定量分析方法。色谱柱为Eclipse plus C18 柱(4.6 mm×250 mm,5 μm),流动相为乙腈-0.25%高氯酸水溶液( 体积比为30∶70),流量为1.0 mL/min,检测波长为210 nm,柱温为25℃。奥曲肽的质量浓度在4.38~219 μg/mL 范围内与色谱峰面积成良好的线性关系,相关系数为0.999 9,检出限为1.1 ng,定量限为2.19 ng。测定结果的相对标准偏差为0.26%~0.46% (n=5),加标回收率为97.41%~100.26%。该方法简便、快速、准确,适用于奥曲肽原料药与制剂的定量分析。
Abstract:A method for the quantitative analysis of octreotide by high performance liquid chromatography was
established. The chromatographic column was Eclipse plus C18 (4.6 mm×250 mm, 5 μm), the mobile phase was
acetonitrile-0.25% perchloric acid aqueous solution (30∶70, v/v), the flow rate was 1.0 mL/min, the detection wave length
was 210 nm, and the column temperature was 25℃. The mass concentration of octreotide had a good linear relationship with
the peak area in the range of 4.38-219 μg/mL, the correlation coefficient was 0.999 9, the detection limit was 1.1 ng, and
the quantitative limit was 2.19 ng. The relative standard deviations of determination rusults were 0.26%-0.46% (n=5), and
the recoveries of standard addition samples were 97.41%-100.26%. This method is simple, rapid, accurate, and it is suitable
for the quantitative analysis of octreotide.
关键词:奥曲肽;高效液相色谱法;定量分析
Keywords:octreotide; high performance liquid chromatography; quantitative analysis
本文引用格式:
向童欣,黄永鹏,唐慧,等. 高效液相色谱法定量分析奥曲肽[J]. 化学分析计量,2020,29(2):79-82.
XIANG T X,HUANG Y P,TANG H,et al. Quantitative analysis of octreotide by high performance liquid chromatography[J]. Chemical analysis and meterage,2020,29(2):79-82.
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