收藏我们   ENGLISH  
论文查询
范围:  
提 交
《化学分析计量》 2019年第06期
DOI:10.3969/j.issn.1008-6145.2019.06.001
液相色谱-同位素稀释质谱法测定婴幼儿配方奶粉中黄曲霉毒素M1
魏敏1,李兴佳1,周剑2,柴艳兵1,张耀广1,李飞1
1. 君乐宝乳业集团农业农村部乳品质量安全控制重点实验室,石家庄 050021;2. 中国农业科学院农业质量标准与检测技术研究所农业农村部农产品质量安全重点实验室,北京 100081 
Determination of aflatoxin M1 in infant formula milk powder by liquid chromatography isotope dilution mass spectrometry
WEI Min1, LI Xingjia1, ZHOU Jian2, CHAI Yanbing1, ZHANG Yaoguang1, LI Fei1
1. Junlebao Dairy Group, Shijiazhuang Key Laboratory of Dairy Quality and Safety Control, Ministry of Agriculture and Villages,Shijiazhuang 050021, China; 2. Key Laboratory of AgrifoodSafety and Quality, Ministry of Agriculture and Rural Affairs, Institute ofQuality Standard and Testing Technology for Agro-Products of CAAS, Beijing 100081, China 
摘要:建立液相色谱-同位素稀释质谱法测定婴幼儿配方奶粉中黄曲霉毒素M1基体标准物质研制的定值方法。采用单点逼近法,以13C17-AFTM1 为内标,乙腈为溶剂,经免疫亲和柱净化,采用单点逼近法测定婴幼儿配方奶粉中黄曲霉毒素M1 的含量。黄曲霉毒素M1 的质量浓度在0.05~5.0 ng/mL 范围内与色谱峰面积线性关系良好,线性相关系数为0.9993,平均回收率为102.0%。该方法稳定性好,操作简单,可以作为婴幼儿配方奶粉中黄曲霉毒素M1基体标准物质研制的定值方法。  
Abstract:A method for the determination of aflatoxin M1 in infant formula milk powder by liquid chromatography -isotope dilution mass spectrometry was established. The content of aflatoxin M1 in infant formula milk powder was determined by single point approximation method with 13C17-AFTM1 as internal standard, acetonitrile was used purified by as solvent and immunoaffinity column. The mass concentration of aflatoxin M1 in the range of 0.05-5.0 ng/mL has a good linear relationship with the peak area of chromatographic spectrometry. The linear correlation coefficient was 0.9993, and the average recovery was 102.0%. The method has good stability and simple operation, and can be used as a fixed value method for the development of aflatoxin M1 matrix reference material in infant formula milk powder.  
关键词:婴幼儿配方奶粉;黄曲霉毒素M1; 基体标准物质;定值方法 
Keywords:infant formula milk powder; aflatoxin M1; matrix reference material; determination method 
基金:国家重点研发计划项目(SQ2018YFC1604302) 
本文引用格式:
魏敏,李兴佳,周剑,等. 液相色谱- 同位素稀释质谱法测定婴幼儿配方奶粉中黄曲霉毒素M1[J]. 化学分析计量,2019,28(6):1-5.
WEI M,LI X J,ZHOU J. Research on accurate measurement of aflatoxin M1 in infant formula[J]. Chemical analysis and meterage,2019,28(6):1-5. 

全文:
相似文章:
  实现标准物质量值溯源性的初步探讨
1996年第02期
《化学分析计量》杂志社 版权所有,未经授权禁止复制或者建立镜像
地址:山东省济南市天桥区田庄东路3号 邮编:250031
电话:0531-85878132 85878148 85878224 85878278 传真:0531-85947355
鲁ICP备05018524号-6