二十碳四烯酸甲酯标准样品的研制
晋文慧123,张怡评13,方华13,陈伟珠13,谢全灵1,洪专13
1.自然资源部第三海洋研究所,福建厦门 361005; 2.华侨大学化工学院,福建厦门 361021; 3. 福建省海洋生物资源开发利用协同创新中心,福建厦门 361005
Development of arachidonate methyl ester certified reference material
晋文慧123,张怡评13,方华13,陈伟珠13,谢全灵1,洪专13
1. Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China;2. Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, China; 3. Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China
摘要:依据GB/T 15000.3-2008工作导则,研制二十碳四烯酸甲酯标准样品。以二十碳四烯酸油粗品为原料,制备高纯二十碳四烯酸甲酯样品,通过红外、质谱、核磁等方法对二十碳四烯酸甲酯样品进行结构鉴定,并进行均匀性、稳定性检验。采用8家实验室进行联合定值,对定值结果进行数据分析。二十碳四烯酸甲酯标准样品均匀性和稳定性良好,其定值结果为99.28%,相对扩展不确定度为0.14%(k=2)。所制得的二十碳四烯酸甲酯样品满足标准样品工作导则的要求,可用于有关二十碳四烯酸甲酯的检测以及相关产品的质量控制。
Abstract:According to the directives for the work of reference materials GB/T 15000.3-2008, arachidonate methyl ester certified reference material was prepared. Arachidonate methyl ester was prepared from crude eicostadienoic acid oil. Its chemical structure was identified by IR, MS and NMR, and its homogeneity and stability were systematically checked. A cooperative certification was conducted by 8 laboratories, and the results of certification for arachidonate methyl ester were analyzed. The homogeneity and stability of arachidonate methyl ester reference material were good. The fixed value was 99.28%, and the relative expanded uncertainty was 0.14%(k=2). The reference material of arachidonate methyl ester conforms to directives for the work of reference materials, and it could be used for method validation and quality control of regarding products.
关键词:二十碳四烯酸甲酯标准样品;均匀性;稳定性;定值;不确定度
Keywords:arachidonate methyl ester certified reference material; homogeneity; stability; certification; uncertainty
基金:国家海洋局第三海洋研究所基本科研业务费(NO. 海三科2016041) ;厦门海洋高技术产业基地创业创新共享服务平台项目(16PFW008SF15) ;海洋中试技术研发与检测公共服务平台建设(Bhsfs009) ;厦门海洋研究开发院共建项目(K170103)
本文引用格式:
晋文慧,张怡评,方华,等. 二十碳四烯酸甲酯标准样品的研制[J]. 化学分析计量,2019,28(2):1-5.
JIN W H,ZHANG Y P,FANG H,et al. Development of arachidonate methyl ester certified reference material[J]. Chemical analysis and meterage,2019,28(2):1-5.
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