气相色谱-质谱法测定人体气味中4种脂肪酸
龙成生,吴德华,王辛,陈然然
公安部南京警犬研究所,南京 210012
Determination of 4 Fatty Acids in Human Body Odor with GC-MS
龙成生,吴德华,王辛,陈然然
Nanjing Police Dog Research Institute, Ministry of Public Security, Nanjing 210012, China
摘要:建立搅拌棒吸附萃取-气相色谱-质谱法测定人体气味中正十二烷酸、正十三烷酸、正十四烷酸、正十六烷酸4种脂肪酸的方法。利用PDMS 涂层搅拌吸附棒直接采集人体气味,采用热脱附程序升温进样方式气相色谱法测定人体气味中4 种脂肪酸。以7-十三酮为内标,建立了4种脂肪酸的内标定量分析方法,线性范围为20~180 ng,相关系数r2为0.9920~0.9975,4种脂肪酸的检出限为3.2~7.5 ng。在40 ng加标水平下,样品加标回收率为90%~105%,测定结果的相对标准偏差为2.7%~4.0%(n=5)。该方法已应用于人体气味化学组分分析,满足分析要求。
Abstract:A method based on stir bar sorption extraction(SBSE)-GC-MS was developed for determination of dodecanoic acid, tridecanoic acid, tetradecanoic acid and hexadecanoic acid in human body odor. 4 fat acids in human body odor were extracted directly by using stir bar coated with PDMS, then injected into GC/MS by means of the thermal desorption injector. 7-tridecanone was used as the internal standard for quantitative analysis. The calibration curves were linear in the range of 20-180 ng and the linear correlation coefficients were 0.9920-0.9975. The detection limits of four fat acids was 3.2-7.5 ng. In 40 ng addition level, the recoveries of 4 acids were 90%-105%, the relative standard deviation of determination results was 2.7%-4.0%(n=5). The method has been used for analysis of fat acids in human body odor, and meet the analysis requirement.
关键词:气相色谱-质谱法;人体气味;脂肪酸;搅拌棒吸附;热脱附
Keywords:gas chromatography-mass spectrometry; human body odor; fatty acids; stir bar sorption extraction; thermal desorption
基金:2015年公安部科技强警基础工作专项(2015GABJC33)
本文引用格式:
龙成生,吴德华,王辛,等.气相色谱-质谱法测定人体气味中4种脂肪酸[J].化学分析计量,2017,26(3):37-41 .
LONG C S,WU D H,WANG X,et al.Determination of 4 fatty acids in human body odor with GC-MS[J]. Chemical analysis and meterage,2017,26(3):37- 41.
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