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《化学分析计量》 2016年第06期
DOI:10.3969/j.issn.1008-6145.2016.06.006
超高效液相色谱法测定虾青素
陈伟珠,张怡评,晋文慧,方华,洪专
国家海洋局第三海洋研究所,国家海洋局海洋生物资源综合利用中心,福建省海洋生物资源开发利用协同创新中心,福建厦门 361005 
Determination of Astaxanthin by Ultra Performance Liquid Chromatography
陈伟珠,张怡评,晋文慧,方华,洪专
Third Institute of Oceanography, State Oceanic Administration, People Republic of China; Engineering Research Center of Marine Biological Resource Comprehensive Utilization, State Oceanic Administration; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen 361005, China 
摘要:建立超高效液相色谱法快速检测虾青素的方法。采用UPLC BEH C8色谱柱(50 mm ×2.1 mm,1.7 μm),考察了流动相、流量及柱温对虾青素样品分离的影响,确定了最佳色谱条件:等度洗脱,流动相为甲醇-水(体积比为75∶25),流量为0.5 mL/min,柱温为40℃,检测波长为475 nm。虾青素的质量浓度在0.2~10.0 μg/mL范围内与其色谱峰面积呈良好的线性关系,线性相关系数r=0.9988,检出限(S/N=3)为0.1 μg/mL,定量限(S/N=10)为0.2 μg/mL。测定结果的相对标准偏差为0.41%(n=6),加标回收率为105.8%~110.3%。该方法快速、简单、可靠、灵敏、重复性好,可用于虾青素有关样品的快速检测。 
Abstract:A ultra performance liquid chromatography(UPLC) method for determination of astaxanthin was developed. The determination of astaxanthin was performed on a UPLC BEH C8 column (50 mm×2.1 mm, 1.7 μm). The influence of mobile phase, flow rate and column temperature on the separation of astaxanthin was comprehensively studied. The optimal separation condition was as follows: the mobile phase was methanol-water (volume ratio was 75∶25) with a isocratic elution profile and flow rate of 0.5 mL/min, the detection wavelength was 475 nm, the column temperature was 40℃. The mass concertration of astaxanthin has good linear ralationship with the chromatographic peak area in the range of 0.2-10.0 μg/mL, the correlation coefficient r was 0.9988. The detection limit was 0.1 μg/mL (S/N=3). The quantitation detection limit was 0.2 μg/mL(S/N=10). The relative standard deviation of determination results was 0.41%(n=6), and the recovery was 105.8%-110.3%. The method is rapid, simple, reliable, and sensitive with a good reproducibility, it can be used for the determination of astaxanthin. 
关键词:虾青素;超高效液相色谱法;快速测定;杂质分离 
Keywords:astaxanthin; ultra-performance liquid chromatography (UPLC); rapid determination; separation of impurity 
基金:福建省科技计划项目(2015N0009)等 
本文引用格式:
陈伟珠,张怡评,晋文慧,等.超高效液相色谱法测定虾青素[J].化学分析计量,2016,25(6):26-29 .
Chen Weizhu,Zhang Yiping,Jin Wenhui,et al.Determination of Astaxanthin by Ultra Performance Liquid Chromatography[J]. Chemical analysis and meterage,2016,25(6):26-29. 

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