加速溶剂萃取和凝胶渗透色谱净化GC-MS法测定烟熏腊肉中16种多环芳烃
周蕾1,陈溪2,袁明珠1,高颖1
鲅鱼圈出入境检验检疫局,辽宁营口 115007;大连出入境检验检疫局,辽宁大连 116000
Determination of 16 Kinds of Polycyclic Aromatic Hydrocarbons in Smoked Bacon by ASE Coupled with GPC and GC-MS
周蕾1,陈溪2,袁明珠1,高颖1
1. Bayuquan Entry–Exit Inspection & Quarantine Bareau, Yingkou 115007, China; 2. Dalian Entry–Exit Inspection & Quarantine Bareau, Dalian 116000, China
摘要:采用ASE萃取、GPC净化和浓缩,建立GC-MS法快速检测烟熏腊肉中16种多环芳烃(PAHs)的分析方法。将烟熏腊肉与硅藻土充分混合后,放到萃取池中加速溶剂萃取,在优化仪器条件下测定,16种PAHs的线性范围为0.1~4 ng/mL,线性相关系数r为0.9853~0.9999。方法检出限在0.172~0.233 μg/kg之间,加标回收率为60.3%~93.2%,测定结果的相对标准偏差为4.06%~11.60%(n=6)。该方法检测快速,准确度高,重现性好,适合于烟熏腊肉中16种PAHs的同时测定。
Abstract:A method was developed for the rapid determination of 16 kinds of polycyclic aromatic hydrocarbons (PAHs) in smoked bacon by gas chromatography-mass spectrometry. ASE extract, GPC purification and enrichment were used. After smoked bacon and diatomite being mixed, then the sample was put in the extract pond to accelerate solvent extraction, and detected under the optimal instrument conditions. The linear range of 16 PAHs was 0.1-4 ng/mL with correlation coefficients of 0.9853-0.9999. The detection limit of the method ranged from 0.172 μg/kg to 0.233 μg/kg, the recoveries were 60.3%-93.2%, and the relative standard deviations of determination results were 4.06%-11.60% (n=6). The method is quick, has high accuracy and good reproducibility, so it is suitable for the simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) in smoked bacon simultaneously.
关键词:烟熏腊肉;多环芳烃;加速溶剂萃取;凝胶渗透色谱;气相色谱
Keywords:smoked bacon; polycyclic aromatic hydrocarbons; accelerated solvent extraction; gel permeation chromatography; gas chromatography-mass spectrometry
基金:辽宁出入境检验检疫局科研项目
本文引用格式:
周蕾,陈溪,袁明珠,等.加速溶剂萃取和凝胶渗透色谱净化GC-MS法测定烟熏腊肉中16种多环芳烃[J].化学分析计量,2016,25(1):15-18.
.ZHOU L,CHEN X,YUAN M Z,et al. Determination of 16 Kinds of Polycyclic Aromatic Hydrocarbons in Smoked Bacon by ASE Coupled with GPC and GC-MS[J]. Chemical analysis and meterage,2016,25(1):15-18.
相似文章: