海藻糖标准样品的研制
陈晖12,方华1,张怡评1,陈伟珠1,洪专1,易瑞灶1
1. 国家海洋局第三海洋研究所,福建厦门 361005; 2. 厦门大学海洋与地球学院,近海海洋环境科学国家重点实验室,福建厦门 361005
Development of Trehalose Certified Reference Material
陈晖12,方华1,张怡评1,陈伟珠1,洪专1,易瑞灶1
1.The Third Institute of Oceanography of the State Oceanic Administration, Xiamen 361005, China; 2.College of Ocean &Earth Science, State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China
摘要:研制了海藻糖国家标准样品。以食品级海藻糖粗品为原料,纯化制备海藻糖,采用红外光谱(IR)、质谱和核磁共振谱(NMR)以及单晶衍射等方法进行结构确证。样品分装成400瓶后,采用离子色谱法进行均匀性、稳定性检验和定值分析。从样品中随机抽取15瓶进行均匀性检验,经F检验表明,在95%的置信区间范围内,样品均匀性良好。在40℃下,经过24个月稳定性考察,结果表明样品稳定性良好。标准样品经国内8家具有分析资质的实验室进行协同定值,并评定了定值结果的不确定度,海藻糖标准样品定值结果为99.72%,扩展不确定度为0.26%(k=1.96)。该标准样品达到国家标准样品的技术要求,可用于有关海藻糖的方法校正和质量控制。
Abstract:Trehalose certified reference material was developed. Trehalose was purified from the crude product. The structure of trehalose certified reference material was comfirmed by infrared spectroscopy (IR), mass spectrometry, NRM and XRD. Trehalose was divided into 400 bottles. The homogeneity and stability testing and quantitative analysis were evaluated by ion chromatography method. According to analysis procedure of homogeneity, 15 bottles of sample were randomly taken from 400 bottles, and the results were validated by F-test statistical methods. The stability inspection was carried on the long-term(24 months), and the results indicated that the period for trehalose storage was 24 months at 40℃. A cooperative certification was conducted by 8 qualified laboratories. The certified purity value of the reference material of trehalose was 99.72% with relative expaned uncertainty of 0.26%(k=1.96). The reference material can conform to the technical requirement of the certified reference material, and it can be used for validation and quality control regarding trehalose.
关键词:海藻糖;标准样品;离子色谱法;定值;不确定度
Keywords:trehalose; certified reference material; ion chromatography; certification; uncertainty
基金:福建省科技计划项目
本文引用格式:
陈晖,方华,张怡评,等.海藻糖标准样品的研制[J].化学分析计量,2015,24(1):1-4.
CHEN H,FANG H,ZHANG Y P,et al.Development of Trehalose Certified Reference Material[J]. Chemical analysis and meterage,2015,24(1):1-4.
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